IHOP brain injury


Overexpression of MBD2 in Glioblastoma Maintains Epigenetic Silencing and Inhibits the Antiangiogenic Function of the Tumor Suppressor Gene BAI1.

Zhu D, Hunter SB, Vertino PM, Van Meir EG
Authors’ Affiliations: Laboratory of Molecular Neuro-Oncology, Departments of Neurosurgery, Pathology and Laboratory Medicine, Radiation Oncology, and Hematology and Medical Oncology, School of Medicine, and Winship Cancer Institute, Emory University, Atlanta, Georgia.

Brain angiogenesis inhibitor 1 (BAI1) is a putative G protein-coupled receptor with potent antiangiogenic and antitumorigenic properties that is mutated in certain cancers. BAI1 is expressed in normal human brain, but it is frequently silenced in glioblastoma multiforme. In this study, we show that this silencing event is regulated by overexpression of methyl-CpG-binding domain protein 2 (MBD2 [?]), a key mediator of epigenetic gene regulation, which binds to the hypermethylated BAI1 gene promoter. In glioma cells, treatment with the DNA demethylating agent 5-aza-2′-deoxycytidine (5-Aza-dC) was sufficient to reactivate BAI1 expression. Chromatin immunoprecipitation showed that MBD2 [?] was enriched at the promoter of silenced BAI1 in glioma cells and that MBD2 [?] binding was released by 5-Aza-dC treatment. RNA interference-mediated knockdown of MBD2 [?] expression led to reactivation of BAI1gene expression and restoration of BAI1 functional activity, as indicated by increased antiangiogenic activity in vitro and in vivo. Taken together, our results suggest that MBD2 [?] overexpression during gliomagenesis may drive tumor growth by suppressing the antiangiogenic activity of a key tumor suppressor. These findings have therapeutic implications because inhibiting MBD2 [?] could offer a strategy to reactivate BAI1 and suppress glioma pathobiology. Cancer Res; 71(17); 5859-70. �2011 AACR.

Cancer Res. (2011)
PMID: 21724586

Advertisements

About garyskeete

ASHWORTH MEDICINE-Professional Medical Assisting, Doctor of Science,Legal Assistant Diploma BSc Criminal Justice
This entry was posted in Uncategorized. Bookmark the permalink.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s