IHOP Pubmed shock protiens

Recombinant Lactobacillus plantarum induces immune responses to cancer testis antigen NY-ESO-1 and maturation of dendritic cells

Anne Mobergslien, Vlada Vasovic, Geir Mathiesen, Lasse Fredriksen, Phuong Westby, Vincent G.H. Eijsink, Qian Peng and Mouldy Sioud
a Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital, Norwayb Department of Pathology, Oslo University Hospital, Norwayc Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Ås. Norway

Human Vaccines & Immunotherapeutics

Published online: 17 Jul 2015

DOI: 10.1080/21645515.2015.1056952

Given their safe use in humans and inherent adjuvanticity, Lactic Acid Bacteria may offer several advantages over other mucosal delivery strategies for cancer vaccines. The objective of this study is to evaluate the immune responses in mice after oral immunization with Lactobacillus (L) plantarum WCFS1 expressing a cell-wall anchored tumour antigen NY-ESO-1. And to investigate the immunostimulatory potency of this new candidate vaccine on human dendritic cells (DCs). L. plantarum displaying NY-ESO-1 induced NY-ESO-1 specific antibodies and T-cell responses in mice. By contrast, L. plantarum displaying either heat shock protein-27 or galectin-1, two conserved proteins, did not induce immunity, suggesting that immune tolerance to self-proteins cannot be broken by oral administration of L. plantarum. With respect to immunomodulation, immature DCs incubated with wild type or L. plantarum-NY-ESO-1 upregulated the expression of co-stimulatory molecules and secreted a large amount of interleukin (IL)-12, TNF-α, but not IL-4. Moreover, they upregulated the expression of immunosuppressive factors such as IL-10 and indoleamine 2,3-dioxygenase. Although L. plantarum-matured DCs expressed inhibitory molecules, they stimulated allogeneic T cells in-vitro. Collectively, the data indicate that L. plantarum-NY-ESO-1 can evoke antigen-specific immunity upon oral administration and induce DC maturation, raising the potential of its use in cancer immunotherapies.

Key Words: Lactobacillus plantarum, oral immunization, tumour antigens, dendritic cells, adjuvants
Abbreviations: APC, antigen presenting cells, DC, dendritic cells, HPV, human papilloma virus, HSP, heat shock protein, iDC, immature DCs, IDO, indoleamine 2,3-dioxygenase, Ig, immunoglobulin, IL, interleukin, LAB, lactic acid bacteria, L, lactobacillus, mDC, mature DC, PD, programmed cell death, Th, T helper
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About garyskeete

ASHWORTH MEDICINE-Professional Medical Assisting, Doctor of Science,Legal Assistant Diploma BSc Criminal Justice PhD Computational Neuroscience MD DSC Epigenetics
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