IHOP allergy and immunology pubmed


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Brain Res. 2015 Jul 30. pii: S0006-8993(15)00576-4. doi: 10.1016/j.brainres.2015.07.033. [Epub ahead of print]
Galectin-1 suppresses methamphetamine induced neuroinflammation in Human brain microvascular endothelial cells: Neuroprotective role in maintaining Blood brain barrier integrity.
Parikh N1, Aalinkeel R1, Reynolds JL1, Nair BB1, Sykes DE1, Mammen MJ1, Schwartz SA1, Mahajan SD2.
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Abstract
Methamphetamine (Meth) abuse can lead to the breakdown of the blood-brain-barrier (BBB) integrity leading to compromised CNS function. The role of Galectins in the angiogenesis process in tumor-associated endothelial cells (EC) is well established; however no data are available on the expression of Galectins in normal human brain microvascular endothelial cells and their potential role in maintaining BBB integrity. We evaluated the basal gene/protein expression levels of Galectin-1, -3 and -9 in normal primary human brain microvascular endothelial cells (BMVEC) that constitute the BBB and examined whether Meth altered Galectin expression in these cells, and if Galectin-1 treatment impacted the integrity of an in-vitro BBB. Our results showed that BMVEC expressed significantly higher levels of Galectin-1 as compared to Galectin-3 and -9. Meth treatment increased Galectin-1 expression in BMVEC. Meth induced decrease in TJ proteins ZO-1, Claudin-3 and adhesion molecule ICAM-1 was reversed by Galectin-1. Our data suggests that Galectin-1 is involved in BBB remodeling and can increase levels of TJ proteins ZO-1 and Claudin-3 and adhesion molecule ICAM-1 which helps maintain BBB tightness thus playing a neuroprotective role. Galectin-1 is thus an important regulator of immune balance from neurodegeneration to neuroprotection, which makes it an important therapeutic agent/ target in the treatment of drug addiction and other neurological conditions.
Copyright © 2015. Published by Elsevier B.V.
KEYWORDS:
Blood Brain Barrier; Galectin-1; Human Brain Microvascular endothelial cells; Methamphetamine; Oxidative stress; Proinflammatory cytokines; Tight Junctions; Transendothelial electrical resistance
PMID: 26236024 [PubMed – as supplied by publisher]

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About garyskeete

ASHWORTH MEDICINE-Professional Medical Assisting, Doctor of Science,Legal Assistant Diploma BSc Criminal Justice
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